Investigation of fluorescence of JC-1 in solution and in cells using spectrofluorimetry, microscopy and flow cytometry reveals that excitation at 405 nm wavelength, now available on standard instruments, produces signals from aggregate fluorescence with considerably less spillover from dye monomer fluorescence than can be obtained using 488 nm excitation. In this study, we show that in practice this approach is not optimal for monitoring mitochondrial behavior. JC-1 fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers. The cyanine dye JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimi- dazolylcarbocyanine iodide) facilitates discrimination of energized and deenergized mitochondria because the normally green fluorescent dye forms red fluorescent aggregates when concentrated in energized mitochondria in response to their higher membrane potential. Cytometry- and microscopy-based analyses, in combination with fluorescent probes, are widely used to study mitochondrial behavior related to cellular pathways, most notably – apoptosis. 2.Mitochondrial membrane potential provides a valuable indicator of cells’ health and functional status. This tutorial will introduce you to FlowJo and to the 6 steps involved in analyzing a basic immunophenotyping experiment. How do I use FlowJo to analyze my flow cytometry data? You can use FlowJo to analyze all of your flow cytometry data - regardless of the cytometer used to collect your data files.
How do I contact FlowJo? USA: (800) 366-6045 International: 1 54 Basic Tutorial Introduction FlowJo software is used for the analysis of flow cytometry data.
3 STEP 3: Try the High Dimensional Data Analysis More. 2 STEP 2: Special Platforms#N#Now that you’ve gotten a handle on how FlowJo works and what types of reports it can generate.
What should I learn first when using FlowJo? What should I learn about first? 1 STEP 1: Getting Started with the Demo Data#N#If you’re a first time user of FlowJo software you should begin by. FlowJo presents a graphical display as well as information about each generation in the subset. Info about Flowjo Instruction How does flowflowjo work? FlowJo models proliferation data obtained using cell tracking dyes such as CFSE.
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